沙门菌非编码小RNA RyhB研究进展

doi:10.11843/j.issn.0366-6964.2019.01.003

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (1): 21-27.doi: 10.11843/j.issn.0366-6964.2019.01.003

沙门菌非编码小RNA RyhB研究进展

陈斌杰^1,2, 王亨^1,2, 陈艳飞^1,2, 潘夏雨³, 袁天梅³, 朱国强^1,2*, 孟霞^1,2*

1. 扬州大学兽医学院, 扬州 225009;
2. 江苏省动物重要疫病与人兽共患病防控协同创新中心, 扬州 225009;
3. 广西园丰牧业集团股份有限公司, 钦州 535400

收稿日期:2018-07-04 出版日期:2019-01-23 发布日期:2019-01-23
通讯作者: 孟霞,主要从事病原菌致病机理研究,E-mail:mengxia_1@126.com;朱国强,主要从事预防兽医学、病原微生物和免疫学研究,E-mail:yzgqzhu@yzu.edu.cn
作者简介:陈斌杰(1993-),男,广东饶平人,硕士生,主要从事病原微生物致病机制研究,E-mail:BinjieChen@126.com
基金资助:
国家重点研发计划（2017YFD0500203；2016YFD0500905）；国家自然科学基金（31101826；31672579）；江苏省高校自然科学研究项目（14KJB230002）；兽医生物技术国家重点实验室开放课题（SKLVBF201509）；扬州市自然科学基金青年科技人才项目（YZ2014019）；江苏高校优势学科建设工程资助项目（PAPD）；扬州市科技计划项目-现代农业（YZ2017054）

Research Progress on Small Non-Coding RNA RyhB of Salmonella

CHEN Binjie^1,2, WANG Heng^1,2, CHEN Yanfei^1,2, PAN Xiayu³, YUAN Tianmei³, ZHU Guoqiang^1,2*, MENG Xia^1,2*

1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
2. Jiangsu Co-Innovation for Important Animal Infectious Diseases and Zoonose, Yangzhou 225009, China;
3. Guangxi YUANFENG Animal Husbandry Group Co. LTD, Qinzhou 535400, China

Received:2018-07-04 Online:2019-01-23 Published:2019-01-23

摘要/Abstract

摘要：

非编码小RNA（small non-coding RNA，sRNA）是一类基因组中被转录但不翻译成蛋白质的RNA分子，可在转录后水平调控基因表达。与蛋白质介导的调控系统不同，当细菌遇到不利的生长环境时，sRNA介导的调控可对环境变化做出快速应答。沙门菌RyhB-1和RyhB-2是两种相似性较高的sRNA，通过碱基互补配对方式，在调控因子作用下共同或单独调控靶基因表达。铁匮乏时，RyhB-1和RyhB-2可促进沙门菌摄取铁元素、限制胞内非必需含铁蛋白生成以及加快铁硫蛋白的储存，是沙门菌在转录后水平调控铁稳态的主要元件。此外，当沙门菌遭遇氧化应激、缺氧或酸性环境等不利环境胁迫时，RyhB可分别控制活性氧自由基的生成、平衡硝酸盐等无机物稳态、调节细菌运动性以及沙门菌毒力等应对环境变化。本文就沙门菌RyhB生理特征及其调控机制和功能进行阐述，以期为后续沙门菌RyhB的研究提供指导信息。

Abstract:

Small non-coding RNA (sRNA) is a type of RNA molecule that is transcribed but not translated into protein in the genome and controls gene expression at the post-transcriptional level. Unlike protein-mediated regulation system, sRNA-mediated regulation can respond to environmental changes quickly when bacteria confront adverse growth environment. RyhB-1 and RyhB-2 are two highly homologous sRNAs in Salmonella, which regulate target genes expression singly or together with the help of regulating factors by base pairing. RyhB-1 and RyhB-2 are major components of Salmonella iron homeostasis regulation at post-transcriptional level by increasing the iron uptake, limiting non-essential iron-utilizing proteins synthesis and accelerating the storage of iron-sulfur protein when iron is scarce. In addition, when Salmonella encounters various environmental stresses such as oxidative stress, hypoxia or acidic environment, RyhB can also control the production of reactive oxygen species, balance the stability of inorganic substances such as nitrate, and regulate bacterial motility and Salmonella virulence to cope with environmental changes. This paper is concerned with the physiological characteristics, regulatory mechanisms and functions of Salmonella RyhB to provide guidance for the subsequent study of RyhB.

中图分类号:

S852.612

陈斌杰, 王亨, 陈艳飞, 潘夏雨, 袁天梅, 朱国强, 孟霞. 沙门菌非编码小RNA RyhB研究进展[J]. 畜牧兽医学报, 2019, 50(1): 21-27.

CHEN Binjie, WANG Heng, CHEN Yanfei, PAN Xiayu, YUAN Tianmei, ZHU Guoqiang, MENG Xia. Research Progress on Small Non-Coding RNA RyhB of Salmonella[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 21-27.

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沙门菌非编码小RNA RyhB研究进展

Research Progress on Small Non-Coding RNA RyhB of Salmonella

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